MSiReader is an open-source vendor-neutral Matlab application to view and perform data analysis of Mass Spectrometry Imaging (MSI) data. A standalone version of MSiReader that does not require a Matlab license is also provided.  Those who are unfamiliar with the Matlab language will have little difficulty navigating the user-friendly interface and users with Matlab programming experience can adapt and customize MSiReader for their own needs.
Because binning is not performed on the imaging data until requested by the user, high resolving power data (small m/z increment) can be viewed and processed as long as the data set size does not exceed memory resources (RAM).